The MECP2 Polyclonal Antibody (CAB0707) is a valuable tool for researchers studying MECP2, a key protein involved in neurological development and function. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By specifically binding to the MECP2 protein, this antibody enables precise detection and analysis in various cell types, making it an excellent choice for studies in neurobiology and genetic disorders.MECP2 is a critical regulator of gene expression in the brain, with mutations in the MECP2 gene linked to Rett Syndrome, a severe neurological disorder primarily affecting girls.
Understanding the function of MECP2 is essential for unraveling the molecular mechanisms underlying neurodevelopmental disorders and potentially identifying new therapeutic targets. The MECP2 Polyclonal Antibody offers researchers a reliable tool for investigating the role of MECP2 in normal brain development and disease, paving the way for advances in clinical research and treatment strategies.
Antibody Name:
Anti-MECP2 Antibody [KO Validated]
Antibody SKU:
CAB0707
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IHC
Reactivity:
Human, Mouse
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MECP2 (NP_004983.1).
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
NCBI Summary:
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13Â Â
NCBI Protein Information:
methyl-CpG-binding protein 2
UniProt Protein Name:
Methyl-CpG-binding protein 2
Protein Family:
Methyl-CpG-binding protein
UniProt Gene Name:
MECP2Â Â
UniProt Entry Name:
MECP2_HUMAN
Western blot analysis of extracts of various cell lines, using MECP2 antibody (CAB0707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Western blot analysis of extracts from normal (control) and MECP2 Rabbit pAb knockout (KO) 293T cells, using MECP2 Rabbit pAb antibody (CAB0707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human gastric cancer using MECP2 antibody (CAB0707) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MECP2 antibody (CAB0707) at dilution of 1:100 (40x lens).
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