The MECOM Polyclonal Antibody (CAB5674) is a valuable tool for researchers studying the MECOM protein, which is involved in various cellular processes, including hematopoiesis and leukemogenesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.MECOM, also known as EVI1, is a transcription factor that plays a critical role in regulating gene expression in hematopoietic stem cells and myeloid progenitors.
Dysregulation of MECOM has been linked to leukemia and other hematologic malignancies, making it a key target for cancer research.By binding specifically to the MECOM protein, this polyclonal antibody enables the detection and analysis of MECOM in different cell types, providing researchers with valuable insights into its function and implications in disease. Its versatility and specificity make it an essential tool for studies in cancer biology and hematopoiesis.
Product Name:
MECOM Rabbit Polyclonal Antibody
SKU:
CAB5674
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MECOM (NP_004982.2).
The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2122
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using MECOM Rabbit pAb (CAB5674) at 1:2500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
