The ME3 Polyclonal Antibody (CAB15787) is a crucial tool for researchers studying the function of ME3, a key enzyme involved in mitochondrial metabolism. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human ME3 protein, making it ideal for use in Western blotting and immunohistochemistry applications.ME3 is a key player in the tricarboxylic acid cycle, where it catalyzes the conversion of malate to pyruvate, a critical step in energy production.
Dysregulation of ME3 has been linked to various metabolic disorders, making it a promising therapeutic target for conditions such as diabetes and obesity. By utilizing the ME3 Polyclonal Antibody, researchers can gain insights into the role of ME3 in metabolic pathways and potentially uncover novel treatment strategies for metabolic diseases.
Product Name:
ME3 Rabbit Polyclonal Antibody
SKU:
CAB15787
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 505-604 of human ME3 (NP_001014811.1).
Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
10873
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ME3 Rabbit pAb (CAB15787) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
