The ME1 Polyclonal Antibody (CAB3956) is a valuable tool for researchers studying ME1, a key enzyme involved in cellular metabolism and energy production. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it an ideal choice for Western blot applications.ME1 is essential for the conversion of malate to pyruvate in the mitochondria, playing a crucial role in the TCA cycle and fatty acid synthesis. Dysregulation of ME1 has been implicated in various diseases, including cancer, diabetes, and metabolic disorders, making it a promising target for therapeutic intervention.
By using the ME1 Polyclonal Antibody, researchers can confidently detect and analyze ME1 expression in different cell types, providing valuable insights into its function and dysregulation in disease states. This antibody is a valuable tool for studies in metabolism, cancer research, and drug development aimed at targeting ME1 for therapeutic purposes.
Product Name:
ME1 Rabbit Polyclonal Antibody
SKU:
CAB3956
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ME1 (NP_002386.1).
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Purification Method:
Affinity purification
Gene ID:
4199
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ME1 antibody (CAB3956) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
