The ME1 Polyclonal Antibody (ABM40238) is a valuable tool for researchers studying the ME1 protein, which plays a crucial role in various cellular processes, including metabolism and energy production. This antibody, produced in rabbits, has been rigorously validated for use in Western blot applications with human samples. Its high specificity and sensitivity make it a reliable choice for detecting and quantifying ME1 in different cell types and tissues.ME1, also known as malic enzyme 1, is an enzyme involved in the citric acid cycle and is essential for the production of NADPH, a key molecule in cellular redox balance and biosynthesis.
Dysregulation of ME1 has been associated with metabolic disorders, cancer, and other diseases, highlighting its importance as a therapeutic target and biomarker for various conditions.By using the ME1 Polyclonal Antibody, researchers can gain valuable insights into the role of ME1 in cellular metabolism and disease progression. Its versatility and reliability make it an invaluable tool for studies in biochemistry, cancer biology, and metabolic research.
Product Name:
[KD Validated] ME1 Polyclonal Antibody
SKU:
CAB23068
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 472-572 of human ME1. (NP_002386.1).
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Purification Method:
Affinity purification
Gene ID:
4199
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and ME1 knockdown (KD) MCF7 cells, using [KD Validated] ME1 Rabbit pAb (CAB23068) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
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