The MDM2 Monoclonal Antibody (CAB23388) is a cutting-edge research tool designed for scientists studying the MDM2 protein, a key regulator of cell growth and division. This monoclonal antibody, generated using advanced techniques, is specifically engineered to target MDM2 in human samples, offering high specificity and reliability in experiments.MDM2 is a crucial protein in the cellular pathway that controls the activity of the tumor suppressor protein p53, which plays a pivotal role in preventing the development of cancer. Dysregulation of MDM2 can lead to uncontrolled cell growth and proliferation, characteristic of cancer cells. By targeting MDM2 with this monoclonal antibody, researchers can explore the mechanisms underlying cancer development and potentially identify new therapeutic targets for cancer treatment.
Validated for use in Western blot applications, the MDM2 Monoclonal Antibody (CAB23388) allows for the precise detection and quantification of MDM2 protein levels in various cell types and tissues. Its high reactivity and specificity make it an invaluable tool for studies in oncology, molecular biology, and drug discovery, providing essential insights into the role of MDM2 in cancer pathogenesis.Unlock the potential of your research with the MDM2 Monoclonal Antibody (CAB23388) and delve into the intricate mechanisms of cancer biology.
Product Name:
MDM2 Monoclonal Antibody
SKU:
CAB23388
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 101-200 of human MDM2 (NP_001354919.1).
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Purification Method:
Affinity purification
Gene ID:
4193
Clone Number:
ARC60296
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from U2OS cells, using MDM2 Rabbit mAb (CAB23388) at 1:2000 dilution.U2OS cells were treated by Nutlin 3a(10μM ) at 37℃ for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
