The MDH2 Polyclonal Antibody (CAB13516) is a valuable tool for researchers studying MDH2, a key enzyme involved in cellular metabolism. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the MDH2 protein, researchers can accurately detect and analyze its expression in various cell types, making this antibody ideal for studies in metabolism, bioenergetics, and cancer research.MDH2, also known as malate dehydrogenase 2, plays a crucial role in the citric acid cycle and oxidative phosphorylation, processes vital for energy production in cells.
Dysregulation of MDH2 has been implicated in various diseases, including cancer and metabolic disorders. By understanding the function and regulation of MDH2, researchers can gain insights into disease mechanisms and potentially develop targeted therapies.Overall, the MDH2 Polyclonal Antibody (CAB13516) is a valuable tool for researchers interested in studying the role of MDH2 in cellular metabolism and disease pathogenesis. Its high specificity and sensitivity make it an essential component of any research project involving MDH2.
Product Name:
MDH2 Rabbit Polyclonal Antibody
SKU:
CAB13516
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_005909.2).
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4191
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MDH2 antibody (CAB13516) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
