The MDH1 Polyclonal Antibody (CAB7563) is a valuable tool for researchers studying MDH1, a key enzyme involved in the citric acid cycle and energy metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.MDH1, also known as malate dehydrogenase 1, plays a crucial role in converting malate to oxaloacetate, a key step in the citric acid cycle that generates energy for cellular processes. Dysregulation of MDH1 has been implicated in various diseases, including cancer and metabolic disorders, making it an important target for research.
By using the MDH1 Polyclonal Antibody, researchers can detect and analyze MDH1 expression in different cell types, providing valuable insights into its role in cellular metabolism and potential implications for disease. This antibody is an essential tool for studies in biochemistry, cell biology, and cancer research, advancing our understanding of MDH1 function and its potential as a therapeutic target.
Product Name:
MDH1 Rabbit Polyclonal Antibody
SKU:
CAB7563
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MDH1 (NP_005908.1).
This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
Purification Method:
Affinity purification
Gene ID:
4190
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MDH1 antibody (CAB7563) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
