The MDC1 Polyclonal Antibody (CAB8358) is a valuable tool for researchers studying MDC1, a key protein involved in DNA damage response and repair pathways. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting MDC1 in human samples, making it ideal for use in Western blot applications.MDC1, also known as Mediator of DNA Damage Checkpoint 1, plays a crucial role in the recruitment and assembly of DNA repair factors at sites of DNA damage, ensuring the maintenance of genomic stability. Dysregulation of MDC1 has been implicated in various diseases, including cancer and genetic disorders, highlighting its importance in genomic maintenance.
By utilizing the MDC1 Polyclonal Antibody, researchers can investigate the expression and localization of MDC1 in different cell types and conditions, providing insights into the mechanisms underlying DNA damage response and potential therapeutic targets for diseases associated with genomic instability. This antibody is a valuable tool for studies in molecular biology, genomics, and cancer research.
Product Name:
MDC1 Rabbit Polyclonal Antibody
SKU:
CAB8358
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
Purification Method:
Affinity purification
Gene ID:
9656
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MDC1 Rabbit pAb (CAB8358) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 5s.
