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MDC1 Rabbit Polyclonal Antibody (CAB8358)

SKU:
CAB8358
Product Type:
Antibody
Applications:
WB
IHC
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 2 Anti-MDC1 Antibody CAB8358
  • Cell Cycle Antibodies 2 Anti-MDC1 Antibody CAB8358
  • Cell Cycle Antibodies 2 Anti-MDC1 Antibody CAB8358
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MDC1 Rabbit Polyclonal Antibody
SKU:CAB8358
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
Sequence:MEDT QAID WDVE EEEE TEQS SESL RCNV EPVG RLHI FSGA HGPE KDFP LHLG KNVV GRMP DCSV ALPF PSIS KQHA EIEI LAWD KAPI LRDC GSLN GTQI LRPP KVLS PGVS HRLR DQEL ILFA DLLC QYHR LDVS LPFV SRGP LTVE ETPR VQGE TQPQ RLLL AEDS EEEV DFLS ERRM VKKS RTTS SSVI VPES DEEG HSPV LGGL GPPF AFNL NSDT DVEE GQQP ATEE ASSA ARRG ATVE AKQS EAEV VTEI QLEK DQPL VKER DNDT KVKR GAGN GVVP AGVI LERS QPPG EDSD TDVD DDSR PPGR PAEV HLER AQPF GFID SDTD AEEE RIPA TPVV IPMK KR
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:NFBD1; MDC1
Positive Sample:Jurkat,LO2
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:227kDa
Observed MW:270kDa

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Purification Method:Affinity purification
Gene ID:9656
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using MDC1 Rabbit pAb (CAB8358) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 5s.

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