The MCU Polyclonal Antibody (CAB16281) is a specialized research tool designed for studying the Mitochondrial Calcium Uniporter (MCU) protein, a crucial player in calcium homeostasis within mitochondria. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the MCU protein, researchers can accurately detect and analyze its expression in various cell types, offering valuable insights into mitochondrial function and calcium signaling pathways.The MCU protein is essential for maintaining mitochondrial calcium levels, which in turn regulate processes such as ATP production, cell metabolism, and cell death.
Dysregulation of MCU has been implicated in various diseases, including neurodegenerative disorders, cardiac dysfunction, and cancer. By studying the role of MCU using this antibody, researchers can gain a deeper understanding of mitochondrial calcium dynamics and its implications for health and disease.Overall, the MCU Polyclonal Antibody (CAB16281) is a valuable tool for researchers conducting studies in the fields of mitochondrial biology, cellular signaling, and disease mechanisms. Its specificity and reliability make it an essential component of any research project aiming to unravel the complexities of MCU function and its impact on cellular physiology.
Product Name:
MCU Rabbit Polyclonal Antibody
SKU:
CAB16281
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 51-170 of human MCU (NP_612366.1).
Enables calcium channel activity; identical protein binding activity; and uniporter activity. Involved in several processes, including positive regulation of mitochondrial calcium ion concentration; positive regulation of mitochondrial fission; and positive regulation of neutrophil chemotaxis. Acts upstream of or within calcium import into the mitochondrion. Located in mitochondrial inner membrane. Is integral component of mitochondrial inner membrane. Part of uniplex complex.
Purification Method:
Affinity purification
Gene ID:
90550
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using MCU Rabbit pAb(CAB16281) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
