The MCTP2 Polyclonal Antibody (PACO59137) is a valuable tool for researchers studying MCTP2, a protein involved in calcium signaling and synaptic vesicle trafficking. This antibody, generated in rabbits, demonstrates high specificity and sensitivity in detecting MCTP2 in human samples, making it suitable for use in Western blot applications. By binding to the MCTP2 protein, this antibody enables researchers to study its role in various cell types and investigate its potential implications in neurological diseases and synaptic transmission.MCTP2, also known as Multiple C2 Domain Proteins with Two Transmembrane Regions 2, is essential for maintaining proper calcium levels within cells and facilitating the release of neurotransmitters at synapses.
Dysregulation of MCTP2 function has been linked to neurodevelopmental disorders and neurological conditions such as epilepsy and Alzheimer's disease. By understanding the molecular mechanisms underlying MCTP2 activity, researchers can develop targeted therapies that may impact synaptic function and neurotransmission in disease contexts.
IHC image of PACO59137 diluted at 1:300 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO59137 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Might play a role in the development of cardiac outflow tract.
Synonyms:
Multiple C2 and transmembrane domain-containing protein 2, MCTP2
UniProt Protein Function:
MCTP2: Belongs to the MCTP family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 15q26.2
Cellular Component: cytoplasm; endoplasmic reticulum; integral to membrane; membrane; nucleoplasm
Molecular Function:calcium ion binding; calcium-dependent phospholipid binding
