Anti-MCM7 Antibody (CAB1138)

Antibody Name:	Anti-MCM7 Antibody
Antibody SKU:	CAB1138
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-719 of human MCM7 (NP_005907.3).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, HeLa, HL-60, MCF7, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-719 of human MCM7 (NP_005907.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GGVD QSPR GMKI RGNI NICL MGDP GVAK SQLL SYID RLAP RSQY TTGR GSSG VGLT AAVL RDSV SGEL TLEG GALV LADQ GVCC IDEF DKMA EADR TAIH EVME QQTI SIAK AGIL TTLN ARCS ILAA ANPA YGRY NPRR SLEQ NIQL PAAL LSRF DLLW LIQD RPDR DNDL RLAQ HITY VHQH SRQP PSQF EPLD MKLM RRYI AMCR EKQP MVPE SLAD YITA AYVE MRRE AWAS KDAT YTSA RTLL AILR LSTA LARL RMVD VVEK EDVN EAIR LMEM SKDS LLGD KGQT ARTQ RPAD VIFA TVRE LVSG GRSV RFSE AEQR CVSR GFTP AQFQ AALD EYEE LNVW QVNA SRTR ITFV
Gene ID:	4176
Uniprot:	P33993
Cellular Location:	Nucleus
Calculated MW:	44kDa/60kDa/81kDa
Observed MW:	91kDa

Synonyms:

MCM7, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104

Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

UniProt Protein Function:	MCM7: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6- MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR- ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Belongs to the MCM family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA replication; EC 3.6.4.12; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: chromatin; cytoplasm; MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm Molecular Function:ATP-dependent DNA helicase activity; DNA binding; protein binding Biological Process: DNA replication; G1/S transition of mitotic cell cycle; regulation of phosphorylation; response to DNA damage stimulus
NCBI Summary:	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	P33993
NCBI GenInfo Identifier:	20981696
NCBI Gene ID:	4176
NCBI Accession:	P33993.4
UniProt Secondary Accession:	P33993,Q15076, Q96D34, Q96GL1, A4D2A1, A4D2A2, E9PGN9
UniProt Related Accession:	P33993
Molecular Weight:	60,643 Da
NCBI Full Name:	DNA replication licensing factor MCM7
NCBI Synonym Full Names:	minichromosome maintenance complex component 7
NCBI Official Symbol:	MCM7
NCBI Official Synonym Symbols:	MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
NCBI Protein Information:	DNA replication licensing factor MCM7
UniProt Protein Name:	DNA replication licensing factor MCM7
UniProt Synonym Protein Names:	CDC47 homolog; P1.1-MCM3
Protein Family:	DNA replication licensing factor
UniProt Gene Name:	MCM7

	Western blot analysis of extracts of various cell lines, using MCM7 antibody (CAB1138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U-2 OS cells using MCM7 Rabbit pAb (CAB1138) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM7 antibody (CAB1138). Western blot was performed from the immunoprecipitate using MCM7 antibody (CAB1138) at a dilition of 1:500.

Antibodies

Anti-MCM7 Antibody (CAB11325)

MCM7 Antibody (PACO02059)

MCM7 Antibody (PACO10424)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)