Anti-MCM6 Antibody (CAB1955)

Antibody Name:	Anti-MCM6 Antibody
Antibody SKU:	CAB1955
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, HeLa, MCF7, HL-60, NIH/3T3, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SIDR VYSL DDIR RYLL FARQ FKPK ISKE SEDF IVEQ YKHL RQRD GSGV TKSS WRIT VRQL ESMI RLSE AMAR MHCC DEVQ PKHV KEAF RLLN KSII RVET PDVN LDQE EEIQ MEVD EGAG GING HADS PAPV NGIN GYNE DINQ ESAP KASL RLGF SEYC RISN LIVL HLRK VEEE EDES ALKR SELV NWYL KEIE SEID SEEE LINK KRII EKVI HRLT HYDH VLIE LTQA GLKG STEG SESY EEDP YLVV NPNY LLED
Gene ID:	4175
Uniprot:	Q14566
Cellular Location:	Nucleus
Calculated MW:	92kDa
Observed MW:	110kDa

Synonyms:

MCM6, MCG40308, Mis5, P105MCM

Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.

UniProt Protein Function:	MCM6: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Belongs to the MCM family.
UniProt Protein Details:	Protein type:EC 3.6.4.12; Cell cycle regulation Chromosomal Location of Human Ortholog: 2q21 Cellular Component: nucleoplasm; MCM complex; nucleus Molecular Function:ATP-dependent DNA helicase activity; identical protein binding; protein binding; single-stranded DNA binding; ATP binding Biological Process: DNA unwinding during replication; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle Disease: Lactose Intolerance, Adult Type
NCBI Summary:	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]
UniProt Code:	Q14566
NCBI GenInfo Identifier:	2497824
NCBI Gene ID:	4175
NCBI Accession:	Q14566.1
UniProt Secondary Accession:	Q14566,Q13504, Q99859, B2R6H2,
UniProt Related Accession:	Q14566
Molecular Weight:	92,889 Da
NCBI Full Name:	DNA replication licensing factor MCM6
NCBI Synonym Full Names:	minichromosome maintenance complex component 6
NCBI Official Symbol:	MCM6
NCBI Official Synonym Symbols:	Mis5; P105MCM; MCG40308
NCBI Protein Information:	DNA replication licensing factor MCM6; minichromosome maintenance deficient (mis5, S. pombe) 6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe)
UniProt Protein Name:	DNA replication licensing factor MCM6
UniProt Synonym Protein Names:	p105MCM
Protein Family:	DNA replication licensing factor
UniProt Gene Name:	MCM6
UniProt Entry Name:	MCM6_HUMAN

	Western blot analysis of extracts of various cell lines, using MCM6 antibody (CAB1955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human lung cancer using MCM6 antibody (CAB1955) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using MCM6 antibody (CAB1955).
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM6 antibody (CAB1955). Western blot was performed from the immunoprecipitate using MCM6 antibody (CAB1955) at a dilition of 1:1000.

Antibodies	ELISA Kits
MCM6 Antibody (PACO02130)	Human MCM6(minichromosome maintenance complex component 6) ELISA Kit
MCM6 Antibody (PACO10423)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)