The MCM6 Polyclonal Antibody (CAB1955) is a vital tool for researchers studying the minichromosome maintenance (MCM) protein family, specifically MCM6. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding to the MCM6 protein, this antibody enables the precise detection and analysis of MCM6 in various cell types, making it an invaluable asset for studies in molecular biology and cancer research.MCM proteins are essential for DNA replication and cell division, playing a crucial role in maintaining genomic stability. As MCM6 is a key member of this protein family, understanding its functions and regulatory mechanisms is vital for elucidating the molecular events underlying cell proliferation and tumorigenesis.
Moreover, aberrant expression of MCM6 has been implicated in various cancers, making it a promising target for the development of novel diagnostic and therapeutic strategies.By utilizing the MCM6 Polyclonal Antibody (CAB1955), researchers can gain valuable insights into the role of MCM6 in cellular processes, ultimately advancing our knowledge of cancer biology and paving the way for the development of innovative cancer treatments.
Product Name:
MCM6 Rabbit Polyclonal Antibody
SKU:
CAB1955
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
Mis5; P105MCM; MCG40308; MCM6
Positive Sample:
U-251MG,HeLa,MCF7,HL-60,NIH/3T3,Mouse spleen
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
93kDa
Observed MW:
110kDa
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.
Purification Method:
Affinity purification
Gene ID:
4175
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MCM6 Rabbit pAb (CAB1955) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
