The MCM5 Rabbit Monoclonal Antibody (CAB5008) is a versatile tool for researchers studying MCM5, a protein involved in DNA replication and cell cycle progression. This antibody, produced using rabbit monoclonal technology, is highly specific for human samples and is validated for use in various experimental applications, including Western blotting and immunofluorescence.MCM5 is a key component of the minichromosome maintenance (MCM) complex, essential for the initiation and elongation of DNA replication. Dysregulation of MCM5 expression has been linked to various diseases, including cancer, making it a valuable target for investigation in oncology research.
By using the MCM5 antibody, researchers can accurately detect and analyze MCM5 protein levels in different cell types, aiding in the understanding of its role in cellular processes and disease development.Furthermore, the MCM5 Rabbit Monoclonal Antibody offers high sensitivity and specificity, allowing for precise detection of MCM5 expression in biological samples. Its superior performance and reliability make it an indispensable tool for researchers exploring the intricate mechanisms of DNA replication and identifying potential therapeutic targets for cancer treatment.
Product Name:
MCM5 Rabbit Monoclonal Antibody
SKU:
CAB5008
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 635-734 of human MCM5 (P33992).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CDC46; MGORS8; P1-CDC46; MCM5
Positive Sample:
HeLa,MCF7,Mouse spleen,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
82kDa
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Purification Method:
Affinity purification
Gene ID:
4174
Clone Number:
ARC1239
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MCM5 Rabbit mAb (CAB5008) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
