The MCM5 Polyclonal Antibody (CAB5556) is specifically designed for researchers studying the Minichromosome Maintenance Complex Component 5 (MCM5) protein. MCM5 is a key protein involved in DNA replication and cell cycle progression, making it essential for maintaining genomic stability.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the MCM5 protein, researchers can easily detect and analyze its expression in various cell types, aiding in studies related to DNA replication, cell division, and cancer research.
The MCM5 Polyclonal Antibody is a valuable tool for investigating the role of MCM5 in cancer development and progression, as well as exploring its potential as a target for therapeutic interventions. Understanding the function of MCM5 is critical for advancing our knowledge of cell cycle regulation and identifying new strategies for treating cancer and other diseases related to abnormal cell proliferation.
Product Name:
MCM5 Rabbit Polyclonal Antibody
SKU:
CAB5556
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
WB,1:1000 - 1:3000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CDC46; MGORS8; P1-CDC46; MCM5
Positive Sample:
HeLa,Jurkat,SW620,Mouse spleen,Rat thymus
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
100kDa
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Purification Method:
Affinity purification
Gene ID:
4174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MCM5 Rabbit pAb (CAB5556) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
