The MCM5 Polyclonal Antibody (CAB13514) is a valuable tool for researchers studying the MCM5 protein, a key component of the minichromosome maintenance complex involved in DNA replication. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the MCM5 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in cell biology and cancer research.
MCM5 plays a crucial role in DNA replication and cell cycle progression, making it a key target for studying processes like DNA repair, genome stability, and cell proliferation. Dysregulation of MCM5 has been implicated in various diseases, including cancer, making it a promising candidate for therapeutic intervention. By gaining insights into the function and regulation of MCM5, researchers can better understand its role in disease development and potentially identify new strategies for treatment.
Product Name:
MCM5 Rabbit Polyclonal Antibody
SKU:
CAB13514
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CDC46; MGORS8; P1-CDC46; MCM5
Positive Sample:
Raji,HL-60,HeLa,SW620,Mouse kidney,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
100kDa
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Purification Method:
Affinity purification
Gene ID:
4174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MCM5 Rabbit pAb (CAB13514) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
