The MCM4 Rabbit Monoclonal Antibody (CAB9251) is a valuable tool for researchers studying the MCM4 protein, a key component of the DNA replication machinery. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for detecting MCM4 in various sample types, particularly in Western blot applications.MCM4 is essential for DNA replication and cell proliferation, making it a critical target for understanding mechanisms of cell growth and division.
Dysregulation of MCM4 has been associated with cancer development and progression, underscoring its importance in oncology research. By targeting MCM4 with this monoclonal antibody, researchers can investigate its role in DNA replication, cell cycle control, and tumorigenesis, contributing to advancements in cancer biology and potential therapeutic strategies.
Product Name:
MCM4 Rabbit Monoclonal Antibody
SKU:
CAB9251
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 764-863 of human MCM4 (P33991).
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Purification Method:
Affinity purification
Gene ID:
4173
Clone Number:
ARC1491
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MCM4 Rabbit mAb (CAB9251) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
