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MCM2 Rabbit Polyclonal Antibody (CAB1056)

SKU:
CAB1056
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-MCM2 Antibody CAB1056
  • Cell Cycle Antibodies 1 Anti-MCM2 Antibody CAB1056
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MCM2 Rabbit Polyclonal Antibody
SKU:CAB1056
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MCM2 (NP_004517.2).
Sequence:PFEV NMEE TIYQ NYQR IRIQ ESPG KVAA GRLP RSKD AILL ADLV DSCK PGDE IELT GIYH NNYD GSLN TANG FPVF ATVI LANH VAKK DNKV AVGE LTDE DVKM ITSL SKDQ QIGE KIFA SIAP SIYG HEDI KRGL ALAL FGGE PKNP GGKH KVRG DINV LLCG DPGT AKSQ FLKY IEKV SSRA IFTT GQGA SAVG LTAY VQRH PVSR EWTL EAGA LVLA DRGV CLID EFDK MNDQ DRTS IHEA MEQQ SISI SKAG IVTS LQAR CTVI AAAN PIGG RYDP SLTF SENV DLTE PIIS RFDI LCVV RDTV DPVQ DEML ARFV VGSH VRHH PSNK EEEG LANG S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN; MCM2
Positive Sample:SW480,SH-SY5Y,Raji,A-549,HeLa,HL-60,Mouse thymus
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:102kDa
Observed MW:130kDa

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.

Purification Method:Affinity purification
Gene ID:4171
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using MCM2 antibody (CAB1056) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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