The MCM10 Polyclonal Antibody (CAB7199) offered by AssayGenie is a valuable tool for researchers studying the role of MCM10, a crucial protein involved in DNA replication and cell cycle progression. This antibody, produced through immunization of rabbits, exhibits high specificity and sensitivity when used with human samples, making it an ideal choice for Western blotting applications.MCM10 is an essential component of the pre-replication complex, playing a central role in the initiation and elongation phases of DNA replication. Dysregulation of MCM10 has been linked to genomic instability and cancer development, making it a target of interest in oncology research.
By using the MCM10 Polyclonal Antibody, researchers can detect and analyze MCM10 protein levels in various cell types, aiding in the exploration of its functions in both normal and disease states.Overall, the MCM10 Polyclonal Antibody from AssayGenie provides a reliable tool for investigating the molecular mechanisms underlying DNA replication and cell cycle control, with potential implications for cancer biology and therapeutic development.
Product Name:
MCM10 Rabbit Polyclonal Antibody
SKU:
CAB7199
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 616-875 of human MCM10 (NP_877428.1).
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
55388
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using MCM10 antibody (CAB7199) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
