The mCherry Rabbit Polyclonal Antibody (CABE171) is specifically designed for research involving mCherry, a fluorescent protein widely used in cellular imaging and tracking applications. This antibody, raised in rabbits, exhibits high reactivity with mCherry tagged proteins and is validated for use in various experimental techniques such as immunofluorescence, Western blotting, and flow cytometry.mCherry is a versatile tool in cell biology research, allowing for visualization and monitoring of cellular processes in real time. Its bright red fluorescence is easily detectable and compatible with a variety of microscopy systems. The mCherry Rabbit Polyclonal Antibody enables researchers to confidently detect and quantify mCherry expression in different cell types, making it an essential reagent for studies in cell biology, molecular biology, and bioimaging.
Whether investigating protein localization, tracking cell behavior, or studying gene expression, the mCherry Rabbit Polyclonal Antibody provides reliable and specific detection of mCherry tagged proteins, facilitating precise and accurate experimental results. Its broad utility and sensitivity make it a valuable asset for researchers working on a wide range of biological questions and experimental approaches.
Product Name:
mCherry Rabbit Polyclonal Antibody
SKU:
CABE171
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of mCherry tag.
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CT55-mCherry using mCherry Rabbit pAb(AE171) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
