The MCCC2 Polyclonal Antibody (CAB15181) is a valuable tool for researchers studying MCCC2, a key enzyme involved in the mitochondrial pathway of propionate metabolism. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting MCCC2 in human samples, making it an essential reagent for Western blotting applications. By targeting the MCCC2 protein, researchers can effectively analyze its expression and function in various cell types, providing insights into metabolic processes and potential therapeutic targets.
MCCC2 plays a crucial role in propionate catabolism, a process essential for energy production and lipid metabolism. Dysregulation of MCCC2 activity has been linked to metabolic disorders and mitochondrial diseases, highlighting its significance in human health. By investigating the role of MCCC2, researchers can advance our understanding of metabolic pathways and develop targeted interventions for metabolic disorders and related conditions.
Product Name:
MCCC2 Rabbit Polyclonal Antibody
SKU:
CAB15181
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 284-563 of human MCCC2 (NP_071415.1).
This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
64087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MCCC2 Rabbit pAb (CAB15181) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
