The MCAT Polyclonal Antibody (CAB15822) is a valuable tool for researchers studying MCAT, a key enzyme involved in mitochondrial fatty acid synthesis. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the MCAT protein, this antibody allows for precise detection and analysis in various cell types, making it an essential component in studies related to metabolism, lipid synthesis, and mitochondrial function.MCAT is a critical enzyme that catalyzes the conversion of malonyl-CoA to malonyl-ACP during fatty acid biosynthesis in mitochondria.
Dysregulation of MCAT has been associated with metabolic disorders, cardiovascular diseases, and cancer, making it a promising target for therapeutic interventions. By understanding the role of MCAT in cellular energy metabolism, researchers can gain insight into the mechanisms underlying these diseases and potentially develop novel treatment strategies.Overall, the MCAT Polyclonal Antibody offers researchers a reliable tool for investigating the function and regulation of MCAT in various biological processes, paving the way for advancements in metabolic research and drug discovery efforts.
Product Name:
MCAT Rabbit Polyclonal Antibody
SKU:
CAB15822
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 191-390 of human MCAT (NP_775738.3).
The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
27349
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from LO2 cells, using MCAT Rabbit pAb (CAB15822) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
