Anti-MCAM Antibody (CAB1962)
- SKU:
- CAB1962
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-MCAM Antibody (CAB1962)
The MCAM Polyclonal Antibody (CAB1962) is a vital tool for researchers studying MCAM, a cell adhesion molecule involved in various biological processes such as cell migration, neural crest development, and cancer metastasis. This antibody, produced in rabbits, demonstrates high specificity and sensitivity for human samples, making it ideal for use in Western blot applications for the detection and analysis of MCAM protein expression.MCAM, also known as melanoma cell adhesion molecule, is a transmembrane glycoprotein that plays a crucial role in cell-cell interactions and signaling pathways. Its overexpression has been associated with tumor progression and metastasis in various cancers, making it a potential target for cancer therapy and prognosis.
By targeting MCAM with this polyclonal antibody, researchers can further investigate its role in cancer biology and potentially develop novel therapeutic strategies.Overall, the MCAM Polyclonal Antibody (CAB1962) is a valuable tool for scientists looking to unravel the complex functions of MCAM in both normal physiological processes and pathological conditions, particularly in the context of cancer research. Its reliable performance and versatility in various experimental techniques make it an essential reagent for studies aiming to elucidate the molecular mechanisms underlying MCAM-associated phenomena.
| Antibody Name: | Anti-MCAM Antibody |
| Antibody SKU: | CAB1962 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 447-646 of human MCAM (NP_006491.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 447-646 of human MCAM (NP_006491.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VLNL SCEA SGHP RPTI SWNV NGTA SEQD QDPQ RVLS TLNV LVTP ELLE TGVE CTAS NDLG KNTS ILFL ELVN LTTL TPDS NTTT GLST STAS PHTR ANST STER KLPE PESR GVVI VAVI VCIL VLAV LGAV LYFL YKKG KLPC RRSG KQEI TLPP SRKS ELVV EVKS DKLP EEMG LLQG SSGD KRAP GDQG EKYI DLRH |
| Gene ID: | 4162 |
| Uniprot: | P43121 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 57kDa/71kDa |
| Observed MW: | 120kDa |
| Synonyms: | MCAM, CD146, MUC18 |
| Background: |
| UniProt Protein Function: | MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration |
| UniProt Code: | P43121 |
| NCBI GenInfo Identifier: | 85681878 |
| NCBI Gene ID: | 4162 |
| NCBI Accession: | P43121.2 |
| UniProt Secondary Accession: | P43121,O95812, Q59E86, Q6PHR3, Q6ZTR2, |
| UniProt Related Accession: | P43121 |
| Molecular Weight: | 646 |
| NCBI Full Name: | Cell surface glycoprotein MUC18 |
| NCBI Synonym Full Names: | melanoma cell adhesion molecule |
| NCBI Official Symbol: | MCAMÂ Â |
| NCBI Official Synonym Symbols: | CD146; MUC18Â Â |
| NCBI Protein Information: | cell surface glycoprotein MUC18; Gicerin; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen |
| UniProt Protein Name: | Cell surface glycoprotein MUC18 |
| UniProt Synonym Protein Names: | Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146 |
| Protein Family: | Cell surface glycoprotein |
| UniProt Gene Name: | MCAMÂ Â |
| UniProt Entry Name: | MUC18_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using MCAM antibody (CAB1962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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