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Anti-MCAM Antibody (CAB17347)

SKU:
CAB17347
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

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Anti-MCAM Antibody (CAB17347)

The MCAM Polyclonal Antibody (CAB17347) is a valuable tool for researchers studying MCAM, a cell surface molecule known to play a role in cell adhesion and migration processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. Its specificity in binding to the MCAM protein allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of cell biology and cancer research.

MCAM, also known as melanoma cell adhesion molecule, is involved in promoting tumor progression and metastasis in various types of cancer. By targeting MCAM in studies, researchers can gain valuable insights into the mechanisms underlying cancer cell behavior and potentially identify new therapeutic targets for cancer treatment. The MCAM Polyclonal Antibody is a crucial tool for researchers looking to unravel the role of MCAM in cancer biology and advance the development of innovative cancer therapies.

Antibody Name:Anti-MCAM Antibody
Antibody SKU:CAB17347
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 340-560 of human MCAM (NP_006491.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 340-560 of human MCAM (NP_006491.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VNYV SDVR VSPA APER QEGS SLTL TCEA ESSQ DLEF QWLR EETG QVLE RGPV LQLH DLKR EAGG GYRC VASV PSIP GLNR TQLV NVAI FGPP WMAF KERK VWVK ENMV LNLS CEAS GHPR PTIS WNVN GTAS EQDQ DPQR VLST LNVL VTPE LLET GVEC TASN DLGK NTSI LFLE LVNL TTLT PDSN TTTG LSTS TASP HTRA NSTS TERK LPEP ESRG V
Gene ID:4162
Uniprot:P43121
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:57kDa/71kDa
Observed MW:118kDa
Synonyms:MCAM, CD146, MUC18
Background:
UniProt Protein Function:MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration

UniProt Code:P43121
NCBI GenInfo Identifier:85681878
NCBI Gene ID:4162
NCBI Accession:P43121.2
UniProt Secondary Accession:P43121,O95812, Q59E86, Q6PHR3, Q6ZTR2,
UniProt Related Accession:P43121
Molecular Weight:646
NCBI Full Name:Cell surface glycoprotein MUC18
NCBI Synonym Full Names:melanoma cell adhesion molecule
NCBI Official Symbol:MCAM  
NCBI Official Synonym Symbols:CD146; MUC18  
NCBI Protein Information:cell surface glycoprotein MUC18; Gicerin; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
UniProt Protein Name:Cell surface glycoprotein MUC18
UniProt Synonym Protein Names:Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146
Protein Family:Cell surface glycoprotein
UniProt Gene Name:MCAM  
UniProt Entry Name:MUC18_HUMAN
Anti-MCAM Antibody (CAB17347)Western blot analysis of extracts of NCI-H460 cells, using MCAM Rabbit pAb (CAB17347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

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