The MBNL1 Rabbit Monoclonal Antibody (CAB5149) is a highly specific tool for researchers studying the function of Muscleblind-like 1 (MBNL1) protein in various biological processes. MBNL1 is known for its role in regulating alternative splicing of pre-mRNA molecules, particularly in the context of myotonic dystrophy, a neuromuscular disorder. This antibody, produced in rabbits, has been validated for use in Western blot and immunofluorescence applications, enabling the detection and visualization of MBNL1 in different cell types and tissues.MBNL1's involvement in RNA processing and splicing makes it a crucial player in the development and progression of diseases like myotonic dystrophy and certain types of cancer.
By targeting MBNL1 with this specific antibody, researchers can gain insights into the protein's function and potential therapeutic interventions that could correct RNA splicing defects associated with disease pathology.Overall, the MBNL1 Rabbit Monoclonal Antibody (CAB5149) is a valuable tool for researchers interested in understanding the molecular mechanisms underlying MBNL1-mediated RNA splicing regulation and its implications in disease development and progression.
Product Name:
MBNL1 Rabbit Monoclonal Antibody
SKU:
CAB5149
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 289-388 of human MBNL1 (Q9NR56).
This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities.
Purification Method:
Affinity purification
Gene ID:
4154
Clone Number:
ARC1199
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MBNL1 Rabbit mAb (CAB5149) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
