The MBD3 Rabbit Monoclonal Antibody (CAB8905) is a cutting-edge tool for researchers studying MBD3, a key player in epigenetic regulation and gene expression. This highly specific antibody, developed using rabbit monoclonal technology, is exceptionally sensitive and reliable for detecting MBD3 in a variety of samples.MBD3 is a member of the MBD (methyl-CpG binding domain) family of proteins, known for their role in interpreting DNA methylation and influencing chromatin structure. MBD3 specifically plays a crucial role in gene silencing and transcriptional regulation, making it a pivotal target in the field of epigenetics research.
The MBD3 Rabbit Monoclonal Antibody is validated for use in various applications, including Western blotting and immunofluorescence, providing researchers with the ability to investigate MBD3 expression and localization in different cell types and experimental conditions. Its superior performance and specificity make it an invaluable tool for studies focused on understanding the intricate mechanisms of epigenetic regulation and their implications in development, disease, and beyond.
Product Name:
MBD3 Rabbit Monoclonal Antibody
SKU:
CAB8905
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MBD3 (O95983).
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene.
Purification Method:
Affinity purification
Gene ID:
53615
Clone Number:
ARC2466
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MBD3 Rabbit mAb (CAB8905) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
