The MBD3 Antibody (CAB2251) is a high-quality antibody developed for reliable detection and analysis of target proteins. MBD3, a member of the Methyl-CpG binding domain (MBD) family, is involved in mediating the effects of DNA methylation on chromatin structure and transcription.This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human MBD3 protein, making it suitable for Western blot applications. By targeting MBD3, researchers can investigate its role in various cellular processes, including development, differentiation, and disease progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MBD3 Antibody
SKU:
CAB2251
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MBD3
Positive Sample:
Jurkat, K-562
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
33kDa
Observed MW:
36kDa
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene.
Purification Method
Affinity purification
Gene ID
53615
RRID
AB_2764252
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MBD3 Rabbit pAb (CAB2251) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
