The MARCH5 Antibody (PACO04656) is a high-quality polyclonal antibody designed for research involving MARCH5, a protein involved in mitochondrial function and cell metabolism. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it a valuable tool for researchers studying mitochondrial dynamics and quality control mechanisms.MARCH5 is a mitochondrial E3 ubiquitin ligase that plays a crucial role in regulating mitochondrial fission and fusion, as well as mitophagy. Dysregulation of MARCH5 has been linked to various diseases, including neurodegenerative disorders and cancer.
By targeting MARCH5 with this antibody, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially develop targeted therapies.The MARCH5 Antibody (PACO04656) is validated for use in Western blot applications, allowing for the detection and analysis of MARCH5 protein levels in different cell types and tissues. Its high specificity and sensitivity make it an essential tool for studies in mitochondrial biology, cell metabolism, and disease mechanisms.
Antibody Name:
MARCH5 Antibody
Antibody SKU:
PACO04656
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
IF:1:200-1:1000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from the Internal region of human 40607.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
MARCH5; RNF153; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153
UniProt Protein Function:
MARCH5: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.Protein type: Membrane protein, multi-pass; Ubiquitin conjugating system; Ligase; EC 6.3.2.19; Membrane protein, integral; EC 6.3.2.-; Ubiquitin ligaseChromosomal Location of Human Ortholog: 10q23.32-q23.33Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; endoplasmic reticulum; integral to membraneMolecular Function: GTPase binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activityBiological Process: protein autoubiquitination; protein polyubiquitination
UniProt Protein Details:
NCBI Summary:
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]
E3 ubiquitin-protein ligase MARCH5; ring finger protein 153; mitochondrial ubiquitin ligase; membrane-associated RING-CH protein V; membrane-associated RING finger protein 5
UniProt Protein Name:
E3 ubiquitin-protein ligase MARCH5
UniProt Synonym Protein Names:
Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153
