The MARCH4 Antibody (PAC04827) is a high-quality polyclonal antibody designed for researchers studying MARCH4, a gene involved in regulating immune responses and protein degradation. This antibody is produced in rabbits and is highly specific for detecting MARCH4 in human samples, making it an excellent tool for Western blotting applications.MARCH4 is a critical component in the process of ubiquitination, which plays a crucial role in protein degradation and turnover within cells. Dysregulation of MARCH4 has been linked to various diseases, including cancer and autoimmune disorders.
By using this antibody, researchers can gain valuable insights into the mechanisms by which MARCH4 influences these conditions.Furthermore, the MARCH4 Antibody is an essential tool for studying the potential therapeutic applications of targeting MARCH4 in the treatment of immune-related diseases. With its high specificity and sensitivity, this antibody enables precise detection and analysis of MARCH4 expression in a variety of cell types, making it an indispensable resource for immunology and cancer research.
Antibody Name:
MARCH4 Antibody
Antibody SKU:
PACO04827
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
IHC:1:100-1:300
Species Reactivity:
Human, Mouse
Immunogen:
synthesized peptide derived from the C-terminal region of human 40606.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
MARCH4; KIAA1399; RNF174; E3 ubiquitin-protein ligase MARCH4; Membrane-associated RING finger protein 4; Membrane-associated RING-CH protein IV; MARCH-IV; RING finger protein 174
UniProt Protein Function:
MARCH4: E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.Protein type: EC 6.3.2.19; EC 6.3.2.-; Membrane protein, multi-pass; Membrane protein, integral; Ubiquitin ligase; Ligase; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 2q35Cellular Component: Golgi stack; trans-Golgi networkMolecular Function: ubiquitin-protein ligase activity
UniProt Protein Details:
NCBI Summary:
MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
