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Anti-MAPK14 Antibody (CAB16822)

SKU:
CAB16822
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
€139 - €419
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Description

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Anti-MAPK14 Antibody (CAB16822)

The MAPK14 Rabbit Polyclonal Antibody (CAB16822) is a powerful tool for researchers studying the MAPK14 protein, a key player in cellular signaling pathways that regulate various biological processes. This antibody, raised in rabbits, has high specificity and sensitivity for detecting MAPK14 in human samples, making it ideal for use in Western blot applications.MAPK14, also known as p38 MAPK, is a critical mediator of cellular responses to stress and inflammation.

It is involved in regulating cell growth, differentiation, and apoptosis, making it a target of interest in cancer research and inflammatory diseases. The MAPK14 Polyclonal Antibody enables researchers to study the role of MAPK14 in different cell types and biological processes, providing valuable insights into its functions and potential therapeutic applications.

Antibody Name:Anti-MAPK14 Antibody
Antibody SKU:CAB16822
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human MAPK14 (NP_620581.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, BxPC-3, LO2, mouse brain, mouse kidney, mouse liver, mouse pancreas, mouse testis, rat kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human MAPK14 (NP_620581.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AQAL AHAY FAQY HDPD DEPV ADPY DQSF ESRD LLID EWKS LTYD EVIS FVPP PLDQ EEME S
Gene ID:1432
Uniprot:Q16539
Cellular Location:
Calculated MW:29kDa/34kDa/35kDa/41kDa
Observed MW:41kDa
Anti-MAPK14 Antibody (CAB16822)Western blot - MAPK14 Polyclonal Antibody (CAB16822)
Synonyms:MAPK14, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, p38 MAPK
Background:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
UniProt Protein Function:P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis
UniProt Protein Details:

Protein type:Protein kinase, CMGC; EC 2.7.11.24; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; p38 subfamily; MAPK/p38 subfamily

Chromosomal Location of Human Ortholog: 6p21.3-p21.2

Cellular Component: nucleoplasm; spindle pole; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function:MAP kinase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; NFAT protein binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; osteoclast differentiation; toll-like receptor 5 signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; transmembrane receptor protein serine/threonine kinase signaling pathway; chondrocyte differentiation; toll-like receptor 4 signaling pathway; cartilage condensation; platelet activation; mitochondrion organization and biogenesis; skeletal muscle development; transcription, DNA-dependent; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; toll-like receptor 2 signaling pathway; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; response to muramyl dipeptide; DNA damage checkpoint; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; toll-like receptor 9 signaling pathway; myoblast cell differentiation involved in skeletal muscle regeneration; apoptosis; cell morphogenesis; chemotaxis; signal transduction; toll-like receptor 10 signaling pathway; lipopolysaccharide-mediated signaling pathway; angiogenesis; MyD88-independent toll-like receptor signaling pathway; positive regulation of erythrocyte differentiation; organelle organization and biogenesis; DNA damage response, signal transduction; positive regulation of myoblast differentiation; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of protein import into nucleus; Ras protein signal transduction; toll-like receptor signaling pathway; innate immune response; positive regulation of muscle cell differentiation; gene expression; cell motility; vascular endothelial growth factor receptor signaling pathway; blood coagulation

NCBI Summary:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:Q16539
NCBI GenInfo Identifier:2499600
NCBI Gene ID:1432
NCBI Accession:Q16539.3
UniProt Secondary Accession:Q16539,O60776, Q13083, Q14084, Q8TDX0, A6ZJ92, A8K6P4 B0LPH0,
UniProt Related Accession:Q16539
Molecular Weight:41,293 Da
NCBI Full Name:Mitogen-activated protein kinase 14
NCBI Synonym Full Names:mitogen-activated protein kinase 14
NCBI Official Symbol:MAPK14  
NCBI Official Synonym Symbols:RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA  
NCBI Protein Information:mitogen-activated protein kinase 14; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; MAP kinase p38 alpha; CSAID-binding protein; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; p38 mitogen activated protein kinase; mitogen-activated protein kinase p38 alpha; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug-binding protein
UniProt Protein Name:Mitogen-activated protein kinase 14
UniProt Synonym Protein Names:Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a
Protein Family:MIP-related peptides
UniProt Gene Name:MAPK14  
UniProt Entry Name:MK14_HUMAN

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