The MAPK1 Polyclonal Antibody (CAB11186) is a valuable tool for researchers investigating the intracellular signaling pathway involving MAPK1, a key regulator of cell growth, differentiation, and survival. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunohistochemistry applications.MAPK1, also known as ERK2, is a member of the MAP kinase family that plays a critical role in various cellular processes, including proliferation, differentiation, and apoptosis. Dysregulation of the MAPK1 pathway has been implicated in a wide range of diseases, from cancer to neurodegenerative disorders.
Therefore, studying the expression and activity of MAPK1 with this antibody can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and specificity, the MAPK1 Polyclonal Antibody (CAB11186) is an essential tool for researchers in the fields of cell biology, cancer research, and drug discovery. Its ability to detect and analyze MAPK1 protein levels in various cell types makes it an indispensable resource for understanding the complex signaling events that govern cellular behavior and disease progression.
Product Name:
[KO Validated] ERK2 Rabbit Polyclonal Antibody
SKU:
CAB11186
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_002736.3).
Sequence:
LNSK GYTK SIDI WSVG CILA EMLS NRPI FPGK HYLD QLNH ILGI LGSP SQED LNCI INLK ARNY LLSL PHKN KVPW NRLF PNAD SKAL DLLD KMLT FNPH K
Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:
41kDa
Observed MW:
42kDa/
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Purification Method:
Affinity purification
Gene ID:
5594
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] ERK2 Rabbit pAb (CAB11186) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
