The MAPK1/MAPK3 Rabbit Polyclonal Antibody (CAB17291) is a valuable tool for researchers studying the MAPK pathway, which plays a critical role in cell signaling and regulation of cellular processes such as proliferation, differentiation, and apoptosis. This antibody is raised in rabbits and highly reactive with human samples, making it an excellent choice for Western blot applications.MAPK1 and MAPK3, also known as ERK2 and ERK1, are key components of the MAPK signaling pathway that is involved in a wide range of cellular functions. Activation of this pathway has been linked to various diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders. By targeting MAPK1 and MAPK3 with this antibody, researchers can investigate their role in disease pathology and potentially develop new therapeutic strategies.
This antibody enables specific detection and analysis of MAPK1 and MAPK3 proteins in various cell types, providing valuable insights into their function and regulation. Its validation for Western blot applications ensures reliable and accurate results, making it a valuable tool for studies in molecular biology, cell signaling, and drug development. Researchers in the fields of oncology, neuroscience, and cardiovascular research will find this antibody particularly useful for advancing their understanding of MAPK signaling.
Product Name:
MAPK1/MAPK3 Rabbit Polyclonal Antibody
SKU:
CAB17291
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAPK1/MAPK3ERK1/2 (P27361/P28482).
caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Calculated MW:
36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW:
44kDa
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Purification Method:
Affinity purification
Gene ID:
5594/5595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MAPK1/MAPK3 Rabbit pAb (CAB17291) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
