The MAPK1/MAPK3 Polyclonal Antibody (CAB16736) is specifically designed for research involving MAPK1 and MAPK3, also known as ERK2 and ERK1, respectively. These proteins are crucial components of the MAPK/ERK signaling pathway, which plays a key role in regulating cell proliferation, differentiation, and survival. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. It binds to both MAPK1 and MAPK3 proteins, allowing for the detection and analysis of their expression levels and activation status in various cell types.
Given the importance of the MAPK/ERK pathway in cancer development, this antibody is ideal for studies in oncology and cancer research. Understanding the activity and regulation of MAPK1 and MAPK3 can provide valuable insights into the mechanisms driving tumorigenesis and potential therapeutic targets for cancer treatment. Overall, the MAPK1/MAPK3 Polyclonal Antibody is a valuable tool for researchers studying cell signaling pathways, cancer biology, and drug development in the field of molecular biology and biomedicine.
Product Name:
MAPK1/MAPK3 Rabbit Polyclonal Antibody
SKU:
CAB16736
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAPK1/MAPK3 (NP_620407.1/NP_002737.2).
Sequence:
LNSK GYTK SIDI WSVG CILA EMLS NRPI FPGK HYLD QLNH ILGI LGSP SQED LNCI INLK ARNY LLSL PHKN KVPW NRLF PNAD SKAL DLLD KMLT FNPH K
caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Calculated MW:
36kDa/38kDa/40kDa/41kDa/43kDa
Observed MW:
41kDa/43kDa
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Purification Method:
Affinity purification
Gene ID:
5594/5595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MAPK1/MAPK3 Rabbit pAb (CAB16736) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
