The MAPK1/MAPK3 Polyclonal Antibody (CAB16686) is a valuable tool for researchers studying the MAPK1 and MAPK3 proteins, which are key components of the mitogen-activated protein kinase (MAPK) signaling pathway. This pathway plays a crucial role in various cellular processes, including cell growth, proliferation, differentiation, and apoptosis.Raised in rabbits, this antibody is highly specific and sensitive for detecting MAPK1 and MAPK3 in human samples. It has been validated for use in Western blot applications, allowing for precise and reliable detection of these important signaling molecules.Researchers in the fields of cell biology, molecular biology, and cancer research will benefit from the use of this antibody in their experiments.
By targeting MAPK1 and MAPK3, researchers can gain insights into the intricate signaling mechanisms that drive cellular processes and potentially uncover new therapeutic targets for diseases such as cancer.In conclusion, the MAPK1/MAPK3 Polyclonal Antibody (CAB16686) is a valuable tool for studying the MAPK signaling pathway and its implications in various biological processes. Its high specificity and sensitivity make it a reliable choice for researchers looking to explore the roles of MAPK1 and MAPK3 in health and disease.
Product Name:
ERK1/ERK2 Rabbit Polyclonal Antibody
SKU:
CAB16686
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK1 / ERK2 (NP_620407.1/NP_002737.2).
Sequence:
LNSK GYTK SIDI WSVG CILA EMLS NRPI FPGK HYLD QLNH ILGI LGSP SQED LNCI INLK ARNY LLSL PHKN KVPW NRLF PNAD SKAL DLLD KMLT FNPH K
caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Calculated MW:
36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW:
42kDa/44kDa
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Purification Method:
Affinity purification
Gene ID:
5594/5595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using ERK1 / ERK2 Rabbit pAb (CAB16686) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
