The MAPK1 Polyclonal Antibody (CAB0229) is a valuable tool for researchers studying MAPK1, a key signaling protein involved in cell growth, differentiation, and survival. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the MAPK1 protein, this antibody enables the detection and analysis of MAPK1 levels in various cell types, making it an excellent choice for studies in cell biology and cancer research.MAPK1, also known as ERK2, is a member of the mitogen-activated protein kinase (MAPK) family and plays a crucial role in transducing extracellular signals to regulate gene expression and cell function.
Dysregulation of MAPK1 signaling has been implicated in various diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders. Therefore, understanding the activity of MAPK1 is essential for developing targeted therapies for these conditions.Overall, the MAPK1 Polyclonal Antibody (CAB0229) provides researchers with a reliable tool to investigate the function of MAPK1 in cellular processes and disease pathways, ultimately contributing to advancements in biomedical research and drug development.
Product Name:
[KO Validated] ERK2 Rabbit Polyclonal Antibody
SKU:
CAB0229
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_002736.3).
Sequence:
LNSK GYTK SIDI WSVG CILA EMLS NRPI FPGK HYLD QLNH ILGI LGSP SQED LNCI INLK ARNY LLSL PHKN KVPW NRLF PNAD SKAL DLLD KMLT FNPH K
Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:
41kDa
Observed MW:
42kDa
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Purification Method:
Affinity purification
Gene ID:
5594
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ERK2 antibody (CAB0229) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
