MAP3K9 Antibody (PACO54330)
- SKU:
- PACO54330
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | MAP3K9 Antibody (PACO54330) |
| Antibody SKU: | PACO54330 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human Mitogen-activated protein kinase kinase kinase 9 protein (864-1098AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat lung tissue. All lanes: MAP3K9 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 122, 124 kDa. Observed band size: 122 kDa. |
 | Immunofluorescence staining of A549 cells with PACO54330 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO54330 diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. |
| Synonyms: | Mitogen-activated protein kinase kinase kinase 9 (EC 2.7.11.25) (Mixed lineage kinase 1), MAP3K9, MLK1 PRKE1 |
| UniProt Protein Function: | MLK1: a TKL kinase of the MLK family. A dual leucine zipper kinase. Expressed in neuronal cells and may act between Rac1/Cdc42 and MKK4 and -7 in death signaling. Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Protein kinase, TKL; TKL group; MLK family; MLK subfamilyChromosomal Location of Human Ortholog: 14q24.2Molecular Function: JUN kinase kinase kinase activity; protein binding; protein homodimerization activity; protein serine/threonine kinase activityBiological Process: activation of JNK activity; protein amino acid autophosphorylation; protein amino acid phosphorylation |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P80192 |
| NCBI GenInfo Identifier: | 116242625 |
| NCBI Gene ID: | 4293 |
| NCBI Accession: | P80192.3 |
| UniProt Secondary Accession: | P80192,Q0D2G7, Q6EH31, Q9H2N5, A3KN85 |
| UniProt Related Accession: | P80192 |
| Molecular Weight: | 123,373 Da |
| NCBI Full Name: | Mitogen-activated protein kinase kinase kinase 9 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 9 |
| NCBI Official Symbol: | MAP3K9Â Â |
| NCBI Official Synonym Symbols: | MLK1; MEKK9; PRKE1Â Â |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 9 |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 9 |
| UniProt Synonym Protein Names: | Mixed lineage kinase 1 |
| Protein Family: | Mitogen-activated protein kinase kinase kinase |
| UniProt Gene Name: | MAP3K9Â Â |
| UniProt Entry Name: | M3K9_HUMAN |
| Antibodies |
| MAP3K9 Antibody (PACO04334) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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