The MAP3K9 Polyclonal Antibody (PAC054330) is a crucial tool for researchers studying MAP3K9, a protein kinase involved in various signal transduction pathways. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot experiments. By binding to the MAP3K9 protein, this antibody enables the detection and analysis of MAP3K9 expression in different cell types, making it an essential resource for investigations in molecular biology and cancer research.MAP3K9 is a key player in cell signaling processes, regulating cell growth, differentiation, and apoptosis.
Its dysregulation has been implicated in the development and progression of various diseases, including cancer and inflammatory disorders. By studying the molecular functions of MAP3K9, researchers can gain insights into its potential as a therapeutic target for treating these conditions.Overall, the MAP3K9 Polyclonal Antibody offers researchers a valuable tool for unraveling the complex functions of MAP3K9 in cellular signaling pathways, paving the way for the development of novel strategies for disease intervention and treatment.
Western Blot. Positive WB detected in: Rat lung tissue. All lanes: MAP3K9 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 122, 124 kDa. Observed band size: 122 kDa.
Immunofluorescence staining of A549 cells with PACO54330 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO54330 diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
MLK1: a TKL kinase of the MLK family. A dual leucine zipper kinase. Expressed in neuronal cells and may act between Rac1/Cdc42 and MKK4 and -7 in death signaling. Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Protein kinase, TKL; TKL group; MLK family; MLK subfamilyChromosomal Location of Human Ortholog: 14q24.2Molecular Function: JUN kinase kinase kinase activity; protein binding; protein homodimerization activity; protein serine/threonine kinase activityBiological Process: activation of JNK activity; protein amino acid autophosphorylation; protein amino acid phosphorylation
