The MAP3K8 Polyclonal Antibody (CAB15623) is specifically designed for researchers studying MAP3K8, a crucial signaling molecule involved in various cellular processes. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the MAP3K8 protein, this antibody allows for the detection and analysis of MAP3K8 in different cell types, making it a valuable tool for studies in molecular biology and cancer research.MAP3K8, also known as tumor progression locus 2 (TPL-2), plays a key role in regulating cell proliferation, differentiation, and survival.
Its involvement in various signaling pathways makes it a promising target for research into diseases such as cancer, inflammation, and immune disorders. Understanding the function of MAP3K8 is essential for developing targeted therapies that modulate its activity and impact these pathological conditions.Overall, the MAP3K8 Polyclonal Antibody provides researchers with a reliable tool for investigating the role of MAP3K8 in cellular processes and disease pathogenesis, paving the way for advancements in targeted therapies and personalized medicine.
Product Name:
MAP3K8 Rabbit Polyclonal Antibody
SKU:
CAB15623
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K8 (NP_005195.2).
COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT; MAP3K8
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol
Calculated MW:
53kDa
Observed MW:
52kDa
This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.
Purification Method:
Affinity purification
Gene ID:
1326
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using MAP3K8 antibody (CAB15623) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
