The MAP3K6 Polyclonal Antibody (PACO55614) is a valuable tool for researchers studying the MAP3K6 protein, which is involved in signaling pathways regulating cell growth, differentiation, and apoptosis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot assays.MAP3K6, also known as transforming growth factor beta-activated kinase 1 (TAK1), plays a crucial role in various cellular processes, including immune response and inflammation. Its dysregulation has been linked to multiple diseases, including cancer, inflammatory disorders, and cardiovascular diseases.
By targeting MAP3K6, researchers can gain insights into the mechanisms underlying these conditions and potentially identify therapeutic strategies.With its ability to specifically bind to MAP3K6, this antibody enables the detection and analysis of the protein in different cell types, making it a valuable tool for studies in molecular biology, biochemistry, and drug development. Understanding the function of MAP3K6 is essential for advancing our knowledge of cellular signaling pathways and developing targeted therapies for a range of diseases.
IHC image of PACO55614 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with PACO55614 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways.
MEKK6: a member of the STE11 protein kinase family. Activates JNK, but not ERK or p38. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Protein type: Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; STE group; STE11 familyChromosomal Location of Human Ortholog: 1p36.11Molecular Function: ATP binding; magnesium ion binding; MAP kinase kinase kinase activityBiological Process: activation of JNK activity; protein amino acid phosphorylation; signal transduction
UniProt Protein Details:
NCBI Summary:
This gene encodes a serine/threonine protein kinase that forms a component of protein kinase-mediated signal transduction cascades. The encoded kinase participates in the regulation of vascular endothelial growth factor (VEGF) expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
