The MAP3K4 Polyclonal Antibody (CAB16918) is a valuable tool for researchers studying MAP3K4, a key protein involved in cell signaling pathways related to cell growth and differentiation. This rabbit polyclonal antibody is highly specific to human MAP3K4 and has been validated for use in Western blot applications. By binding to the MAP3K4 protein, this antibody allows for accurate detection and analysis of MAP3K4 expression in various cell types.MAP3K4, also known as mitogen-activated protein kinase kinase kinase 4, plays a crucial role in regulating cell proliferation, apoptosis, and response to environmental stressors.
Its involvement in multiple signaling cascades makes it a promising target for research in cancer biology, developmental biology, and neuroscience. Understanding the function of MAP3K4 can provide insights into disease mechanisms and potential therapeutic targets for various conditions.Overall, the MAP3K4 Polyclonal Antibody is an essential tool for investigating the role of MAP3K4 in cellular signaling pathways, making it an ideal choice for researchers studying various aspects of cell biology and disease pathology.
Product Name:
MAP3K4 Rabbit Polyclonal Antibody
SKU:
CAB16918
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K4 (NP_005913.2).
The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
4216
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MAP3K4 Rabbit pAb (CAB16918) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
