null

MEKK3 Rabbit Polyclonal Antibody (CAB16058)

SKU:
CAB16058
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 6 Anti-MAP3K3 Antibody CAB16058
  • Cell Biology Antibodies 6 Anti-MAP3K3 Antibody CAB16058
  • Cell Biology Antibodies 6 Anti-MAP3K3 Antibody CAB16058
  • Cell Biology Antibodies 6 Anti-MAP3K3 Antibody CAB16058
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MEKK3 Rabbit Polyclonal Antibody
SKU:CAB16058
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MEKK3 (NP_976226.1).
Sequence:MDEQ EALN SIMN DLVA LQMN RRHR MPGY ETMK NKDT GHSN RQKK HNSS SSAL LNSP TVTT SSCA GASE KKKF LSDV RIKF EHNG ERRI IAFS RPVK YEDV EHKV TTVF GQPL DLHY MNNE LSIL LKNQ DDLD KAID ILDR SSSM KSLR ILLL SQDR NHNS SSPH SGVS RQVR IKAS QSAG DINT IYQP PEPR SRHL SVSS QNPG RSSP PPGY VPER QQHI
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:100 - 1:200
Synonyms:MEKK3; MAPKKK3
Positive Sample:K-562,Mouse brain,Mouse liver,Rat pancreas
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol
Calculated MW:71kDa
Observed MW:71kDa

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification Method:Affinity purification
Gene ID:4215
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using MEKK3 Rabbit pAb (CAB16058) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Related Products