The MAP3K3 Polyclonal Antibody (CAB16058) is a valuable tool for research involving the MAP3K3 protein, a key signaling molecule involved in various cellular processes. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MAP3K3 protein, this antibody enables the sensitive detection and analysis of MAP3K3 expression in different cell types, making it suitable for studies in molecular biology and signal transduction research.MAP3K3, also known as MEKK3, is a crucial component of the mitogen-activated protein kinase (MAPK) signaling pathway, playing a pivotal role in cell growth, differentiation, and survival.
Dysregulation of MAP3K3 has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions. Therefore, studying the function of MAP3K3 is essential for uncovering potential therapeutic targets and developing treatments for these pathological conditions.In summary, the MAP3K3 Polyclonal Antibody (CAB16058) is a powerful tool for researchers studying the MAP3K3 protein and its role in cellular signaling pathways. Its high specificity and reliability make it an essential reagent for investigating the molecular mechanisms underlying disease development and progression.
Product Name:
MEKK3 Rabbit Polyclonal Antibody
SKU:
CAB16058
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MEKK3 (NP_976226.1).
This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
4215
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MEKK3 Rabbit pAb (CAB16058) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
