The MAP1LC3B Polyclonal Antibody (CAB10007) is a valuable tool for researchers studying autophagy, a crucial cellular process involved in maintaining cellular homeostasis and responding to stress. This antibody, generated in rabbits, specifically targets the MAP1LC3B protein, a key player in autophagosome formation and autophagic flux.With its high reactivity to human samples, the MAP1LC3B Polyclonal Antibody is ideal for use in Western blotting applications, enabling researchers to easily detect and analyze MAP1LC3B levels in various cell types.
This makes it a valuable resource for studies in cell biology, cancer research, and neurodegenerative diseases where autophagy dysregulation is implicated.Understanding the role of MAP1LC3B in autophagy pathways is essential for developing targeted therapies and interventions for diseases associated with autophagy dysfunction. By using this antibody, researchers can gain valuable insights into the mechanisms underlying autophagy and its potential as a therapeutic target.
Product Name:
MAP1LC3B Polyclonal Antibody
SKU:
CAB21800
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-50 of human MAP1LC3B (NP_073729.1).
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Purification Method:
Affinity purification
Gene ID:
81631
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells using MAP1LC3B Rabbit pAb (CAB21800) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
