MAP1LC3B Polyclonal Antibody (CAB21800)
- SKU:
- CAB21800
Frequently bought together:
Description
| Product Name: | MAP1LC3B Polyclonal Antibody |
| SKU: | CAB21800 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-50 of human MAP1LC3B (NP_073729.1). |
| Sequence: | MPSE KTFK QRRT FEQR VEDV RLIR EQHP TKIP VIIE RYKG EKQL PVLD KT |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; 3B |
| Positive Sample: | 293T Chloroquine,293T,C6 Chloroquine,C6 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton. |
| Calculated MW: | 14kDa |
| Observed MW: | 14kDa,16kDa |
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
| Purification Method: | Affinity purification |
| Gene ID: | 81631 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from 293T cells using MAP1LC3B Rabbit pAb (CAB21800) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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