The MAP1LC3B Polyclonal Antibody (CAB5601) is a valuable tool for researchers studying autophagy, a crucial cellular process for maintaining cellular homeostasis and eliminating damaged organelles or proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.MAP1LC3B, also known as microtubule-associated protein 1 light chain 3 beta, is a key protein involved in the formation of autophagosomes, the double-membrane vesicles responsible for engulfing cellular components targeted for degradation.
By targeting MAP1LC3B with this antibody, researchers can detect and analyze autophagy activity in various cell types, providing insights into the regulation of this process in normal and disease states.The role of autophagy in health and disease, including cancer, neurodegenerative disorders, and metabolic diseases, has made it an attractive target for drug development. Understanding the regulation of MAP1LC3B and its involvement in autophagy offers potential therapeutic opportunities for modulating this process in various pathological conditions.
Product Name:
LC3B Rabbit Polyclonal Antibody
SKU:
CAB5601
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1).
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Purification Method:
Affinity purification
Gene ID:
81631
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using LC3B Rabbit pAb (CAB5601) at 1:2000 dilution.NIH/3T3 and C6 were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
