The MAP1LC3B Polyclonal Antibody (CAB15592) is a valuable tool for researchers studying autophagy, a cellular process essential for maintaining cellular homeostasis. This antibody, produced in rabbits, specifically targets the MAP1LC3B protein, a key marker for autophagosomes and autophagy initiation.The antibody has been validated for use in a variety of applications, including Western blot, immunofluorescence, and immunohistochemistry, making it versatile for a wide range of experimental needs. Its high specificity ensures accurate detection and analysis of MAP1LC3B in various cell types, allowing for detailed studies in autophagy-related research.
MAP1LC3B is a crucial component of the autophagy machinery, playing a central role in the formation and maturation of autophagosomes. Dysregulation of autophagy has been implicated in numerous diseases, including neurodegenerative disorders, cancer, and metabolic conditions. By studying the activity of MAP1LC3B, researchers can gain insights into the mechanisms of autophagy and its implications for disease pathology, potentially leading to the development of novel therapeutic strategies.
Product Name:
LC3B Polyclonal Antibody
SKU:
CAB15592
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1).
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Purification Method:
Affinity purification
Gene ID:
81631
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of 293T, using LC3B Rabbit pAb (CAB15592) at 1:2000 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
