The MAP1LC3B Polyclonal Antibody (CAB11282) is a vital tool for researchers studying autophagy, a cellular process involved in the degradation and recycling of cellular components. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MAP1LC3B protein, this antibody enables the accurate detection and analysis of autophagy processes in various cell types, making it an essential tool for studies in cell biology, cancer research, and neurodegenerative diseases.
MAP1LC3B, a key player in the autophagy pathway, is crucial for maintaining cellular homeostasis and protecting against various stressors, including nutrient deprivation, infection, and oxidative damage. Dysregulation of autophagy has been implicated in a wide range of diseases, such as cancer, neurodegenerative disorders, and metabolic syndromes. Therefore, understanding the role of MAP1LC3B in autophagy is essential for developing novel therapeutic strategies targeting these diseases and improving patient outcomes.
Product Name:
[KO Validated] LC3B Rabbit Polyclonal Antibody
SKU:
CAB11282
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human LC3B (NP_073729.1).
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Purification Method:
Affinity purification
Gene ID:
81631
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using LC3B Rabbit pAb (CAB11282) at 1:1000 dilution. NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
