The MAP1LC3B Polyclonal Antibody (CAB11280) is a valuable tool for researchers studying autophagy, a crucial cellular process involved in maintaining cell homeostasis and preventing neurodegenerative diseases. This antibody, generated in rabbits, has high specificity for human samples and is validated for use in Western blot and immunohistochemistry applications. MAP1LC3B is a key protein in the autophagy pathway, essential for the formation of autophagosomes and the degradation of cellular components. Dysregulation of autophagy has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic conditions.
By targeting MAP1LC3B, researchers can investigate the role of autophagy in these diseases and develop new therapeutic strategies.This antibody enables precise detection and analysis of MAP1LC3B in a variety of cell types, providing valuable insights into the mechanisms underlying autophagy and its implications for disease pathology. Its superior performance and reliability make it an indispensable tool for studies in cell biology, neuroscience, and drug development. Unlock the potential of autophagy research with the MAP1LC3B Polyclonal Antibody (CAB11280).
Product Name:
LC3B Rabbit Polyclonal Antibody
SKU:
CAB11280
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human LC3B (NP_073729.1).
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Purification Method:
Affinity purification
Gene ID:
81631
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and LC3B knockdown (KD) 293T cells, using [KD Validated] LC3B Rabbit pAb (CAB11280) at 1:1000 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
