The MAP1LC3A Rabbit Monoclonal Antibody is an essential tool for researchers studying autophagy, a cellular process crucial for maintaining cellular homeostasis and eliminating damaged components. This antibody, developed using rabbits as the host species, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications for the detection of MAP1LC3A protein.MAP1LC3A, a key marker for autophagy, is involved in the formation of autophagosomes and serves as a reliable indicator of autophagic activity within cells. By targeting MAP1LC3A, researchers can gain insights into the mechanisms underlying autophagy and its role in various biological processes, including cellular growth, differentiation, and response to stress.
The versatility of the MAP1LC3A Rabbit Monoclonal Antibody allows for its use in a wide range of cell types and experimental settings, making it a valuable tool for studies in cell biology, cancer research, and neurodegenerative diseases. By understanding the regulation and function of MAP1LC3A, researchers can uncover new therapeutic targets and potential treatments for diseases associated with dysregulated autophagy.
Product Name:
MAP1LC3A Rabbit Monoclonal Antibody
SKU:
CAB12319
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (Q9H492).
Sequence:
MPSD RPFK QRRS FADR CKEV QQIR DQHP SKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELV KIIR RRLQ LNPT QAFF LLVN QHSM VSVS TPIA DIYE
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
Purification Method:
Affinity purification
Gene ID:
84557
Clone Number:
ARC2636
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3,C6 cells using MAP1LC3A Rabbit mAb (CAB12319) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 180s.
